Heavy Metal Resistant Bacteria Isolated from Hydrocarbons Contaminated Soil

Document Type : Original Article

Abstract

FOUR bacterial isolates were isolated from hydrocarbons…. contaminated soil from Korea. The isolated bacteria were tested for their heavy metal resistance with different concentrations of Pb2+, Cd2+, Cu2+ and Cr6+ (0-100 mg L-1) on a Tris-minimal medium using agar diffusion method. The result shows that Cu2+ and Pb2+ resistance was the most widespread. None of the tested isolates were tolerated for Cr6+. Specifically, the bacterial isolate Bacillus X4 was multiple metal resistances to Cu2+, Pb2+ and Cd2+ of concentrations 50, 50 and 10 mg L-1, respectively. The resistant bacterium was identified based on 16S rRNA as Bacillus megaterium X4. Results suggest that Bacillus megaterium X4. can be used to remediate heavy metals from hydrocarbons contaminated soils.

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